The topology resulting from the combined chloroplast and nuclear data recovers some morphology-based sections, such as Acanthostemma and Eriostemma, as well as a well-supported Australian/New Guinean clade. pubicalyx, pointing to recent hybridization or the persistence of paralogous copies through a speciation event. carnosa, however, grouped with a sequence of the morphologically similar H. Most ITS clones grouped by species, indicating that speciation in Hoya usually predates ITS duplication. To assess levels of ITS polymorphism, ITS sequences for a third of the accessions were obtained by cloning. We here infer major species groups in the genus based on combined sequences from the chloroplast atpB-rbcL spacer, the trnL region, and nuclear ribosomal DNA ITS region for 42 taxa of Hoya and close relatives.
Hoya (Marsdenieae, Apocynaceae) includes at least 200 species distributed from India to the Pacific Islands.
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